Both dynein and kinesin motors have been implicated in RNA localization in Drosophila oocytes, whereas a type V myosin motor is required for the transport of ASH1 mRNA in budding yeast ( Long et al., 1997 Brendza et al., 2000 Schnorrer et al., 2000 Cha et al., 2002 Duncan and Warrior, 2002 Januschke et al., 2002 St Johnston, 2005). These studies have revealed complex mechanisms in which one motor protein or the coordinated action of a few motor proteins act to direct transport and localization of RNAs to their final destination ( Gagnon and Mowry, 2011). Several recent studies in yeast and Drosophila have illuminated the roles that molecular motors play in the process of RNA localization. In a variety of cell types and species, transport of mRNA to a specific cellular compartment enables localized translation, hence generating asymmetric distribution of proteins that are essential for the establishment and maintenance of cellular polarity and structural asymmetry within the cell ( Holt and Bullock, 2009 Mili and Macara, 2009). A systematic investigation in Drosophila embryos reveals that >70% of endogenous transcripts are localized and the localization of mRNA corresponds to the localization of their encoded proteins ( LГ©cuyer et al., 2007). MRNA localization is a fundamental mechanism for spatial and temporal control of gene expression ( Martin and Ephrussi, 2009). Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes ОІ-actin mRNA and affects cell migration. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo.

We show that KIF11 colocalizes with the ОІ-actin mRNA, and the ability of KIF11 to transport ОІ-actin mRNA is dependent on ZBP1. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of ОІ-actin messenger ribonucleoprotein particles (mRNPs). Although the mechanism of ОІ-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. The length of the protein (amino acid residues) (according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0 and Phobius and predicted transmembrane region(s) (according to MDM) are also reported.ZBP1-modulated localization of ОІ-actin mRNA enables a cell to establish polarity and structural asymmetry. The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. Parent protein classes are in bold font and subclasses are listed under the parent class. The protein classes to which this protein has been assigned are shown if expanding the data in the protein class column. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein. The ENSP identifier links to the Ensembl website for that protein, and the ENST identifier links to the Ensembl website for that transcript. The protein information section displays the alternative protein-coding transcripts (splice variants) encoded by this gene, according to the Ensembl database. Q9H171 (UniProt - Evidence at protein level) Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. This gene encodes a Z-DNA binding protein. If any of the protein products of the gene is linked to a UniProt KB/SWISS-PROT entry, links to the UniProt and the neXtProt databases for these proteins are displayed. The Entrez gene identifier for the gene is also given. The chromosomal and cytoband location of the gene according to Ensembl is reported together with the Ensembl gene identifier and Ensembl database version. The gene information section lists the gene name (HUGO Gene Nomenclature Committee ( HGNC) name if available), any approved gene synonyms, Ensembl gene description, and the Entrez gene summary from the National Center for Biotechnology Information.

Zbp1 gene bioedit